AustralieFrV1, Main, Exploration, bibRecord, 002D45

Genomic analysis of diversity, population structure, virulence, and antimicrobial resistance in Klebsiella pneumoniae, an urgent threat to public health

Identifieur interne : 002D45 ( Main/Exploration ); précédent : 002D44; suivant : 002D46

Genomic analysis of diversity, population structure, virulence, and antimicrobial resistance in Klebsiella pneumoniae, an urgent threat to public health

Auteurs : Kathryn E. Holt [Australie] ; Heiman Wertheim [Royaume-Uni] ; Ruth N. Zadoks [Royaume-Uni] ; Stephen Baker [Viêt Nam] ; Chris A. Whitehouse ; David Dance [Royaume-Uni] ; Adam Jenney [Australie] ; Thomas R. Connor [Royaume-Uni] ; Li Yang Hsu [Singapour] ; Juliëtte Severin [Pays-Bas] ; Sylvain Brisse [France] ; Hanwei Cao [Australie] ; Jonathan Wilksch [Australie] ; Claire Gorrie [Australie] ; Mark B. Schultz [Australie] ; David J. Edwards [Australie] ; Kinh Van Nguyen [Viêt Nam] ; Trung Vu Nguyen [Viêt Nam] ; Trinh Tuyet Dao [Viêt Nam] ; Martijn Mensink ; Vien Le Minh [Viêt Nam] ; Nguyen Thi Khanh Nhu [Viêt Nam, Australie] ; Constance Schultsz [Viêt Nam, Pays-Bas] ; Kuntaman Kuntaman [Indonésie] ; Paul N. Newton [Royaume-Uni] ; Catrin E. Moore [Royaume-Uni] ; Richard A. Strugnell [Australie] ; Nicholas R. Thomson [Royaume-Uni]

Source :

Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 2015.

RBID : PMC:4500264

Descripteurs français

KwdFr :
- Analyse de séquence d'ADN, Animaux, Anti-infectieux (pharmacologie), Dynamique des populations, Gènes bactériens (génétique), Génome bactérien (génétique), Génomique (), Humains, Infection croisée (microbiologie), Infections à Klebsiella (microbiologie), Klebsiella pneumoniae (), Klebsiella pneumoniae (génétique), Klebsiella pneumoniae (pathogénicité), Multirésistance bactérienne aux médicaments (génétique), Phylogénie, Protéines bactériennes (), Protéines bactériennes (génétique), Santé publique (), Santé publique (tendances), Spécificité d'espèce, Variation génétique, Virulence (génétique).
MESH :
- génétique : Gènes bactériens, Génome bactérien, Klebsiella pneumoniae, Multirésistance bactérienne aux médicaments, Protéines bactériennes, Virulence.
- microbiologie : Infection croisée, Infections à Klebsiella.
- pathogénicité : Klebsiella pneumoniae.
- pharmacologie : Anti-infectieux.
- tendances : Santé publique.
- Analyse de séquence d'ADN, Animaux, Dynamique des populations, Génomique, Humains, Klebsiella pneumoniae, Phylogénie, Protéines bactériennes, Santé publique, Spécificité d'espèce, Variation génétique.

English descriptors

KwdEn :
- Animals, Anti-Infective Agents (pharmacology), Bacterial Proteins (classification), Bacterial Proteins (genetics), Cross Infection (microbiology), Drug Resistance, Multiple, Bacterial (genetics), Genes, Bacterial (genetics), Genetic Variation, Genome, Bacterial (genetics), Genomics (methods), Humans, Klebsiella Infections (microbiology), Klebsiella pneumoniae (classification), Klebsiella pneumoniae (genetics), Klebsiella pneumoniae (pathogenicity), Phylogeny, Population Dynamics, Public Health (statistics & numerical data), Public Health (trends), Sequence Analysis, DNA, Species Specificity, Virulence (genetics).
MESH :
- chemical , classification : Bacterial Proteins.
- chemical , genetics : Bacterial Proteins.
- chemical , pharmacology : Anti-Infective Agents.
- classification : Klebsiella pneumoniae.
- genetics : Drug Resistance, Multiple, Bacterial, Genes, Bacterial, Genome, Bacterial, Klebsiella pneumoniae, Virulence.
- methods : Genomics.
- microbiology : Cross Infection, Klebsiella Infections.
- pathogenicity : Klebsiella pneumoniae.
- statistics & numerical data : Public Health.
- trends : Public Health.
- Animals, Genetic Variation, Humans, Phylogeny, Population Dynamics, Sequence Analysis, DNA, Species Specificity.

Abstract

Significance

Klebsiella pneumoniae is rapidly becoming untreatable using last-line antibiotics. It is especially problematic in hospitals, where it causes a range of acute infections. To approach controlling such a bacterium, we first must define what it is and how it varies genetically. Here we have determined the DNA sequence of K. pneumoniae isolates from around the world and present a detailed analysis of these data. We show that there is a wide spectrum of diversity, including variation within shared sequences and gain and loss of whole genes. Using this detailed blueprint, we show that there is an unrecognized association between the possession of specific gene profiles associated with virulence and antibiotic resistance and the differing disease outcomes seen for K. pneumoniae.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4500264

DOI: 10.1073/pnas.1501049112
PubMed: 26100894
PubMed Central: 4500264

Affiliations:

Australie, France, Indonésie, Pays-Bas, Royaume-Uni, Singapour, Viêt Nam

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Genomic analysis of diversity, population structure, virulence, and antimicrobial resistance in <italic>Klebsiella pneumoniae</italic>
, an urgent threat to public health</title>
<author><name sortKey="Holt, Kathryn E" sort="Holt, Kathryn E" uniqKey="Holt K" first="Kathryn E." last="Holt">Kathryn E. Holt</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute,<institution>The University of Melbourne</institution>
, Parkville, VIC 3010, Australia;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>, Parkville, VIC 3010</wicri:regionArea>
<wicri:noRegion>VIC 3010</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff2">Department of Microbiology and Immunology,<institution>The University of Melbourne</institution>
, Parkville, VIC 3010, Australia;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>, Parkville, VIC 3010</wicri:regionArea>
<wicri:noRegion>VIC 3010</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wertheim, Heiman" sort="Wertheim, Heiman" uniqKey="Wertheim H" first="Heiman" last="Wertheim">Heiman Wertheim</name>
<affiliation><nlm:aff id="aff3">Oxford University Clinical Research Unit, Wellcome Trust Major Overseas Programme,<institution>National Hospital for Tropical Diseases</institution>
, Hanoi,<institution>Vietnam</institution>
;</nlm:aff>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff4">Nuffield Department of Clinical Medicine, Centre for Tropical Medicine,<institution>University of Oxford</institution>
, OX3 7BN Oxford,<country>United Kingdom</country>
;</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Zadoks, Ruth N" sort="Zadoks, Ruth N" uniqKey="Zadoks R" first="Ruth N." last="Zadoks">Ruth N. Zadoks</name>
<affiliation><nlm:aff id="aff5">Quality Milk Production Services,<institution>Cornell University</institution>
, Ithaca,<addr-line>NY</addr-line>
 14853;</nlm:aff>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff6">Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical, Veterinary and Life Sciences,<institution>University of Glasgow</institution>
, G12 8QQ Glasgow,<country>United Kingdom</country>
;</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Baker, Stephen" sort="Baker, Stephen" uniqKey="Baker S" first="Stephen" last="Baker">Stephen Baker</name>
<affiliation wicri:level="1"><nlm:aff id="aff7">The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme,<institution>Oxford University Clinical Research Unit</institution>
, Ho Chi Minh City,<country>Vietnam</country>
;</nlm:aff>
<country xml:lang="fr">Viêt Nam</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Whitehouse, Chris A" sort="Whitehouse, Chris A" uniqKey="Whitehouse C" first="Chris A." last="Whitehouse">Chris A. Whitehouse</name>
<affiliation><nlm:aff id="aff8"><institution>United States Army Medical Research Institute of Infectious Diseases</institution>
, Fort Detrick,<addr-line>MD</addr-line>
 21702;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Dance, David" sort="Dance, David" uniqKey="Dance D" first="David" last="Dance">David Dance</name>
<affiliation wicri:level="1"><nlm:aff id="aff4">Nuffield Department of Clinical Medicine, Centre for Tropical Medicine,<institution>University of Oxford</institution>
, OX3 7BN Oxford,<country>United Kingdom</country>
;</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation><nlm:aff id="aff9">Lao-Oxford-Mahosot Hospital Wellcome Trust Research Unit, Microbiology Laboratory,<institution>Mahosot Hospital</institution>
, Vientiane,<country>Lao People’s Democratic Republic</country>
;</nlm:aff>
<wicri:noCountry code="nlm country">Lao People’s Democratic Republic</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Jenney, Adam" sort="Jenney, Adam" uniqKey="Jenney A" first="Adam" last="Jenney">Adam Jenney</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">Department of Microbiology and Immunology,<institution>The University of Melbourne</institution>
, Parkville, VIC 3010, Australia;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>, Parkville, VIC 3010</wicri:regionArea>
<wicri:noRegion>VIC 3010</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff10">Departmemt Infectious Diseases and Microbiology Unit,<institution>The Alfred Hospital</institution>
, Melbourne, VIC 3004, Australia;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>, Melbourne, VIC 3004</wicri:regionArea>
<wicri:noRegion>VIC 3004</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Connor, Thomas R" sort="Connor, Thomas R" uniqKey="Connor T" first="Thomas R." last="Connor">Thomas R. Connor</name>
<affiliation wicri:level="1"><nlm:aff id="aff11">Pathogen Genomics,<institution>Wellcome Trust Sanger Centre</institution>
, CB10 1SA Cambridge,<country>United Kingdom</country>
;</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff12">Cardiff University School of Biosciences,<institution>Cardiff University</institution>
, Cardiff, Wales, CF10 3AX,<country>United Kingdom</country>
;</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Hsu, Li Yang" sort="Hsu, Li Yang" uniqKey="Hsu L" first="Li Yang" last="Hsu">Li Yang Hsu</name>
<affiliation wicri:level="1"><nlm:aff id="aff13">Department of Medicine,<institution>National University Health System</institution>
,<country>Singapore</country>
 119228;</nlm:aff>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Severin, Juliette" sort="Severin, Juliette" uniqKey="Severin J" first="Juliëtte" last="Severin">Juliëtte Severin</name>
<affiliation wicri:level="1"><nlm:aff id="aff14">Department of Medical Microbiology and Infectious Diseases,<institution>Erasmus University Medical Center</institution>
, 3015 CE Rotterdam,<country>The Netherlands</country>
;</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Brisse, Sylvain" sort="Brisse, Sylvain" uniqKey="Brisse S" first="Sylvain" last="Brisse">Sylvain Brisse</name>
<affiliation wicri:level="1"><nlm:aff id="aff15">Microbial Evolutionary Genomics,<institution>Institut Pasteur</institution>
, CNRS, UMR3525, Paris,<country>France</country>
;</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Cao, Hanwei" sort="Cao, Hanwei" uniqKey="Cao H" first="Hanwei" last="Cao">Hanwei Cao</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">Department of Microbiology and Immunology,<institution>The University of Melbourne</institution>
, Parkville, VIC 3010, Australia;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>, Parkville, VIC 3010</wicri:regionArea>
<wicri:noRegion>VIC 3010</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff16"><institution>Peter Doherty Institute, The University of Melbourne</institution>
, Parkville, VIC 3010, Australia;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>, Parkville, VIC 3010</wicri:regionArea>
<wicri:noRegion>VIC 3010</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wilksch, Jonathan" sort="Wilksch, Jonathan" uniqKey="Wilksch J" first="Jonathan" last="Wilksch">Jonathan Wilksch</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">Department of Microbiology and Immunology,<institution>The University of Melbourne</institution>
, Parkville, VIC 3010, Australia;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>, Parkville, VIC 3010</wicri:regionArea>
<wicri:noRegion>VIC 3010</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff16"><institution>Peter Doherty Institute, The University of Melbourne</institution>
, Parkville, VIC 3010, Australia;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>, Parkville, VIC 3010</wicri:regionArea>
<wicri:noRegion>VIC 3010</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Gorrie, Claire" sort="Gorrie, Claire" uniqKey="Gorrie C" first="Claire" last="Gorrie">Claire Gorrie</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute,<institution>The University of Melbourne</institution>
, Parkville, VIC 3010, Australia;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>, Parkville, VIC 3010</wicri:regionArea>
<wicri:noRegion>VIC 3010</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff2">Department of Microbiology and Immunology,<institution>The University of Melbourne</institution>
, Parkville, VIC 3010, Australia;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>, Parkville, VIC 3010</wicri:regionArea>
<wicri:noRegion>VIC 3010</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff16"><institution>Peter Doherty Institute, The University of Melbourne</institution>
, Parkville, VIC 3010, Australia;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>, Parkville, VIC 3010</wicri:regionArea>
<wicri:noRegion>VIC 3010</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Schultz, Mark B" sort="Schultz, Mark B" uniqKey="Schultz M" first="Mark B." last="Schultz">Mark B. Schultz</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute,<institution>The University of Melbourne</institution>
, Parkville, VIC 3010, Australia;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>, Parkville, VIC 3010</wicri:regionArea>
<wicri:noRegion>VIC 3010</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Edwards, David J" sort="Edwards, David J" uniqKey="Edwards D" first="David J." last="Edwards">David J. Edwards</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute,<institution>The University of Melbourne</institution>
, Parkville, VIC 3010, Australia;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>, Parkville, VIC 3010</wicri:regionArea>
<wicri:noRegion>VIC 3010</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Nguyen, Kinh Van" sort="Nguyen, Kinh Van" uniqKey="Nguyen K" first="Kinh Van" last="Nguyen">Kinh Van Nguyen</name>
<affiliation wicri:level="1"><nlm:aff id="aff17"><institution>National Hospital for Tropical Diseases</institution>
, Hanoi,<country>Vietnam</country>
;</nlm:aff>
<country xml:lang="fr">Viêt Nam</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Nguyen, Trung Vu" sort="Nguyen, Trung Vu" uniqKey="Nguyen T" first="Trung Vu" last="Nguyen">Trung Vu Nguyen</name>
<affiliation wicri:level="1"><nlm:aff id="aff17"><institution>National Hospital for Tropical Diseases</institution>
, Hanoi,<country>Vietnam</country>
;</nlm:aff>
<country xml:lang="fr">Viêt Nam</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Dao, Trinh Tuyet" sort="Dao, Trinh Tuyet" uniqKey="Dao T" first="Trinh Tuyet" last="Dao">Trinh Tuyet Dao</name>
<affiliation wicri:level="1"><nlm:aff id="aff17"><institution>National Hospital for Tropical Diseases</institution>
, Hanoi,<country>Vietnam</country>
;</nlm:aff>
<country xml:lang="fr">Viêt Nam</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Mensink, Martijn" sort="Mensink, Martijn" uniqKey="Mensink M" first="Martijn" last="Mensink">Martijn Mensink</name>
<affiliation><nlm:aff id="aff5">Quality Milk Production Services,<institution>Cornell University</institution>
, Ithaca,<addr-line>NY</addr-line>
 14853;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Minh, Vien Le" sort="Minh, Vien Le" uniqKey="Minh V" first="Vien Le" last="Minh">Vien Le Minh</name>
<affiliation wicri:level="1"><nlm:aff id="aff7">The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme,<institution>Oxford University Clinical Research Unit</institution>
, Ho Chi Minh City,<country>Vietnam</country>
;</nlm:aff>
<country xml:lang="fr">Viêt Nam</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation><nlm:aff id="aff18">Division of Infectious Diseases, Department of Medicine,<institution>University of California, San Francisco</institution>
,<addr-line>CA 94118-6215</addr-line>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Nhu, Nguyen Thi Khanh" sort="Nhu, Nguyen Thi Khanh" uniqKey="Nhu N" first="Nguyen Thi Khanh" last="Nhu">Nguyen Thi Khanh Nhu</name>
<affiliation wicri:level="1"><nlm:aff id="aff7">The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme,<institution>Oxford University Clinical Research Unit</institution>
, Ho Chi Minh City,<country>Vietnam</country>
;</nlm:aff>
<country xml:lang="fr">Viêt Nam</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff19">School of Chemistry and Molecular Biosciences,<institution>The University of Queensland</institution>
, Brisbane, QLD 4072,<country>Australia</country>
;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Schultsz, Constance" sort="Schultsz, Constance" uniqKey="Schultsz C" first="Constance" last="Schultsz">Constance Schultsz</name>
<affiliation wicri:level="1"><nlm:aff id="aff7">The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme,<institution>Oxford University Clinical Research Unit</institution>
, Ho Chi Minh City,<country>Vietnam</country>
;</nlm:aff>
<country xml:lang="fr">Viêt Nam</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff24">Academic Medical Center,<institution>University of Amsterdam</institution>
, 1012 WX Amsterdam,<country>The Netherlands</country>
;</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Kuntaman, Kuntaman" sort="Kuntaman, Kuntaman" uniqKey="Kuntaman K" first="Kuntaman" last="Kuntaman">Kuntaman Kuntaman</name>
<affiliation wicri:level="1"><nlm:aff id="aff21">Department of Clinical Microbiology, Dr. Soetomo Academic Hospital – School of Medicine,<institution>Airlangga University</institution>
, Surabaya, Jawa Timur,<country>Indonesia</country>
;</nlm:aff>
<country xml:lang="fr">Indonésie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Newton, Paul N" sort="Newton, Paul N" uniqKey="Newton P" first="Paul N." last="Newton">Paul N. Newton</name>
<affiliation wicri:level="1"><nlm:aff id="aff4">Nuffield Department of Clinical Medicine, Centre for Tropical Medicine,<institution>University of Oxford</institution>
, OX3 7BN Oxford,<country>United Kingdom</country>
;</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation><nlm:aff id="aff9">Lao-Oxford-Mahosot Hospital Wellcome Trust Research Unit, Microbiology Laboratory,<institution>Mahosot Hospital</institution>
, Vientiane,<country>Lao People’s Democratic Republic</country>
;</nlm:aff>
<wicri:noCountry code="nlm country">Lao People’s Democratic Republic</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Moore, Catrin E" sort="Moore, Catrin E" uniqKey="Moore C" first="Catrin E." last="Moore">Catrin E. Moore</name>
<affiliation wicri:level="1"><nlm:aff id="aff4">Nuffield Department of Clinical Medicine, Centre for Tropical Medicine,<institution>University of Oxford</institution>
, OX3 7BN Oxford,<country>United Kingdom</country>
;</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation><nlm:aff id="aff9">Lao-Oxford-Mahosot Hospital Wellcome Trust Research Unit, Microbiology Laboratory,<institution>Mahosot Hospital</institution>
, Vientiane,<country>Lao People’s Democratic Republic</country>
;</nlm:aff>
<wicri:noCountry code="nlm country">Lao People’s Democratic Republic</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Strugnell, Richard A" sort="Strugnell, Richard A" uniqKey="Strugnell R" first="Richard A." last="Strugnell">Richard A. Strugnell</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">Department of Microbiology and Immunology,<institution>The University of Melbourne</institution>
, Parkville, VIC 3010, Australia;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>, Parkville, VIC 3010</wicri:regionArea>
<wicri:noRegion>VIC 3010</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff16"><institution>Peter Doherty Institute, The University of Melbourne</institution>
, Parkville, VIC 3010, Australia;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>, Parkville, VIC 3010</wicri:regionArea>
<wicri:noRegion>VIC 3010</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Thomson, Nicholas R" sort="Thomson, Nicholas R" uniqKey="Thomson N" first="Nicholas R." last="Thomson">Nicholas R. Thomson</name>
<affiliation wicri:level="1"><nlm:aff id="aff11">Pathogen Genomics,<institution>Wellcome Trust Sanger Centre</institution>
, CB10 1SA Cambridge,<country>United Kingdom</country>
;</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff25">Department of Pathogen Molecular Biology,<institution>The London School of Hygiene and Tropical Medicine</institution>
, WC1E 7HT London,<country>United Kingdom</country>
</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">26100894</idno>
<idno type="pmc">4500264</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4500264</idno>
<idno type="RBID">PMC:4500264</idno>
<idno type="doi">10.1073/pnas.1501049112</idno>
<date when="2015">2015</date>
<idno type="wicri:Area/Pmc/Corpus">000227</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000227</idno>
<idno type="wicri:Area/Pmc/Curation">000227</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000227</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000F65</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000F65</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="wicri:Area/PubMed/Corpus">002C04</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002C04</idno>
<idno type="wicri:Area/PubMed/Curation">002B31</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002B31</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002B31</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002B31</idno>
<idno type="wicri:Area/Ncbi/Merge">002744</idno>
<idno type="wicri:Area/Ncbi/Curation">002744</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">002744</idno>
<idno type="wicri:doubleKey">0027-8424:2015:Holt K:genomic:analysis:of</idno>
<idno type="wicri:Area/Main/Merge">002D45</idno>
<idno type="wicri:Area/Main/Curation">002D45</idno>
<idno type="wicri:Area/Main/Exploration">002D45</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Genomic analysis of diversity, population structure, virulence, and antimicrobial resistance in <italic>Klebsiella pneumoniae</italic>
, an urgent threat to public health</title>
<author><name sortKey="Holt, Kathryn E" sort="Holt, Kathryn E" uniqKey="Holt K" first="Kathryn E." last="Holt">Kathryn E. Holt</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute,<institution>The University of Melbourne</institution>
, Parkville, VIC 3010, Australia;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>, Parkville, VIC 3010</wicri:regionArea>
<wicri:noRegion>VIC 3010</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff2">Department of Microbiology and Immunology,<institution>The University of Melbourne</institution>
, Parkville, VIC 3010, Australia;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>, Parkville, VIC 3010</wicri:regionArea>
<wicri:noRegion>VIC 3010</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wertheim, Heiman" sort="Wertheim, Heiman" uniqKey="Wertheim H" first="Heiman" last="Wertheim">Heiman Wertheim</name>
<affiliation><nlm:aff id="aff3">Oxford University Clinical Research Unit, Wellcome Trust Major Overseas Programme,<institution>National Hospital for Tropical Diseases</institution>
, Hanoi,<institution>Vietnam</institution>
;</nlm:aff>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff4">Nuffield Department of Clinical Medicine, Centre for Tropical Medicine,<institution>University of Oxford</institution>
, OX3 7BN Oxford,<country>United Kingdom</country>
;</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Zadoks, Ruth N" sort="Zadoks, Ruth N" uniqKey="Zadoks R" first="Ruth N." last="Zadoks">Ruth N. Zadoks</name>
<affiliation><nlm:aff id="aff5">Quality Milk Production Services,<institution>Cornell University</institution>
, Ithaca,<addr-line>NY</addr-line>
 14853;</nlm:aff>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff6">Institute of Biodiversity, Animal Health and Comparative Medicine, College of Medical, Veterinary and Life Sciences,<institution>University of Glasgow</institution>
, G12 8QQ Glasgow,<country>United Kingdom</country>
;</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Baker, Stephen" sort="Baker, Stephen" uniqKey="Baker S" first="Stephen" last="Baker">Stephen Baker</name>
<affiliation wicri:level="1"><nlm:aff id="aff7">The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme,<institution>Oxford University Clinical Research Unit</institution>
, Ho Chi Minh City,<country>Vietnam</country>
;</nlm:aff>
<country xml:lang="fr">Viêt Nam</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Whitehouse, Chris A" sort="Whitehouse, Chris A" uniqKey="Whitehouse C" first="Chris A." last="Whitehouse">Chris A. Whitehouse</name>
<affiliation><nlm:aff id="aff8"><institution>United States Army Medical Research Institute of Infectious Diseases</institution>
, Fort Detrick,<addr-line>MD</addr-line>
 21702;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Dance, David" sort="Dance, David" uniqKey="Dance D" first="David" last="Dance">David Dance</name>
<affiliation wicri:level="1"><nlm:aff id="aff4">Nuffield Department of Clinical Medicine, Centre for Tropical Medicine,<institution>University of Oxford</institution>
, OX3 7BN Oxford,<country>United Kingdom</country>
;</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation><nlm:aff id="aff9">Lao-Oxford-Mahosot Hospital Wellcome Trust Research Unit, Microbiology Laboratory,<institution>Mahosot Hospital</institution>
, Vientiane,<country>Lao People’s Democratic Republic</country>
;</nlm:aff>
<wicri:noCountry code="nlm country">Lao People’s Democratic Republic</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Jenney, Adam" sort="Jenney, Adam" uniqKey="Jenney A" first="Adam" last="Jenney">Adam Jenney</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">Department of Microbiology and Immunology,<institution>The University of Melbourne</institution>
, Parkville, VIC 3010, Australia;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>, Parkville, VIC 3010</wicri:regionArea>
<wicri:noRegion>VIC 3010</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff10">Departmemt Infectious Diseases and Microbiology Unit,<institution>The Alfred Hospital</institution>
, Melbourne, VIC 3004, Australia;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>, Melbourne, VIC 3004</wicri:regionArea>
<wicri:noRegion>VIC 3004</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Connor, Thomas R" sort="Connor, Thomas R" uniqKey="Connor T" first="Thomas R." last="Connor">Thomas R. Connor</name>
<affiliation wicri:level="1"><nlm:aff id="aff11">Pathogen Genomics,<institution>Wellcome Trust Sanger Centre</institution>
, CB10 1SA Cambridge,<country>United Kingdom</country>
;</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff12">Cardiff University School of Biosciences,<institution>Cardiff University</institution>
, Cardiff, Wales, CF10 3AX,<country>United Kingdom</country>
;</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Hsu, Li Yang" sort="Hsu, Li Yang" uniqKey="Hsu L" first="Li Yang" last="Hsu">Li Yang Hsu</name>
<affiliation wicri:level="1"><nlm:aff id="aff13">Department of Medicine,<institution>National University Health System</institution>
,<country>Singapore</country>
 119228;</nlm:aff>
<country xml:lang="fr">Singapour</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Severin, Juliette" sort="Severin, Juliette" uniqKey="Severin J" first="Juliëtte" last="Severin">Juliëtte Severin</name>
<affiliation wicri:level="1"><nlm:aff id="aff14">Department of Medical Microbiology and Infectious Diseases,<institution>Erasmus University Medical Center</institution>
, 3015 CE Rotterdam,<country>The Netherlands</country>
;</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Brisse, Sylvain" sort="Brisse, Sylvain" uniqKey="Brisse S" first="Sylvain" last="Brisse">Sylvain Brisse</name>
<affiliation wicri:level="1"><nlm:aff id="aff15">Microbial Evolutionary Genomics,<institution>Institut Pasteur</institution>
, CNRS, UMR3525, Paris,<country>France</country>
;</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Cao, Hanwei" sort="Cao, Hanwei" uniqKey="Cao H" first="Hanwei" last="Cao">Hanwei Cao</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">Department of Microbiology and Immunology,<institution>The University of Melbourne</institution>
, Parkville, VIC 3010, Australia;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>, Parkville, VIC 3010</wicri:regionArea>
<wicri:noRegion>VIC 3010</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff16"><institution>Peter Doherty Institute, The University of Melbourne</institution>
, Parkville, VIC 3010, Australia;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>, Parkville, VIC 3010</wicri:regionArea>
<wicri:noRegion>VIC 3010</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wilksch, Jonathan" sort="Wilksch, Jonathan" uniqKey="Wilksch J" first="Jonathan" last="Wilksch">Jonathan Wilksch</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">Department of Microbiology and Immunology,<institution>The University of Melbourne</institution>
, Parkville, VIC 3010, Australia;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>, Parkville, VIC 3010</wicri:regionArea>
<wicri:noRegion>VIC 3010</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff16"><institution>Peter Doherty Institute, The University of Melbourne</institution>
, Parkville, VIC 3010, Australia;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>, Parkville, VIC 3010</wicri:regionArea>
<wicri:noRegion>VIC 3010</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Gorrie, Claire" sort="Gorrie, Claire" uniqKey="Gorrie C" first="Claire" last="Gorrie">Claire Gorrie</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute,<institution>The University of Melbourne</institution>
, Parkville, VIC 3010, Australia;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>, Parkville, VIC 3010</wicri:regionArea>
<wicri:noRegion>VIC 3010</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff2">Department of Microbiology and Immunology,<institution>The University of Melbourne</institution>
, Parkville, VIC 3010, Australia;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>, Parkville, VIC 3010</wicri:regionArea>
<wicri:noRegion>VIC 3010</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff16"><institution>Peter Doherty Institute, The University of Melbourne</institution>
, Parkville, VIC 3010, Australia;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>, Parkville, VIC 3010</wicri:regionArea>
<wicri:noRegion>VIC 3010</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Schultz, Mark B" sort="Schultz, Mark B" uniqKey="Schultz M" first="Mark B." last="Schultz">Mark B. Schultz</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute,<institution>The University of Melbourne</institution>
, Parkville, VIC 3010, Australia;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>, Parkville, VIC 3010</wicri:regionArea>
<wicri:noRegion>VIC 3010</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Edwards, David J" sort="Edwards, David J" uniqKey="Edwards D" first="David J." last="Edwards">David J. Edwards</name>
<affiliation wicri:level="1"><nlm:aff id="aff1">Department of Biochemistry and Molecular Biology, Bio21 Molecular Science and Biotechnology Institute,<institution>The University of Melbourne</institution>
, Parkville, VIC 3010, Australia;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>, Parkville, VIC 3010</wicri:regionArea>
<wicri:noRegion>VIC 3010</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Nguyen, Kinh Van" sort="Nguyen, Kinh Van" uniqKey="Nguyen K" first="Kinh Van" last="Nguyen">Kinh Van Nguyen</name>
<affiliation wicri:level="1"><nlm:aff id="aff17"><institution>National Hospital for Tropical Diseases</institution>
, Hanoi,<country>Vietnam</country>
;</nlm:aff>
<country xml:lang="fr">Viêt Nam</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Nguyen, Trung Vu" sort="Nguyen, Trung Vu" uniqKey="Nguyen T" first="Trung Vu" last="Nguyen">Trung Vu Nguyen</name>
<affiliation wicri:level="1"><nlm:aff id="aff17"><institution>National Hospital for Tropical Diseases</institution>
, Hanoi,<country>Vietnam</country>
;</nlm:aff>
<country xml:lang="fr">Viêt Nam</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Dao, Trinh Tuyet" sort="Dao, Trinh Tuyet" uniqKey="Dao T" first="Trinh Tuyet" last="Dao">Trinh Tuyet Dao</name>
<affiliation wicri:level="1"><nlm:aff id="aff17"><institution>National Hospital for Tropical Diseases</institution>
, Hanoi,<country>Vietnam</country>
;</nlm:aff>
<country xml:lang="fr">Viêt Nam</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Mensink, Martijn" sort="Mensink, Martijn" uniqKey="Mensink M" first="Martijn" last="Mensink">Martijn Mensink</name>
<affiliation><nlm:aff id="aff5">Quality Milk Production Services,<institution>Cornell University</institution>
, Ithaca,<addr-line>NY</addr-line>
 14853;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Minh, Vien Le" sort="Minh, Vien Le" uniqKey="Minh V" first="Vien Le" last="Minh">Vien Le Minh</name>
<affiliation wicri:level="1"><nlm:aff id="aff7">The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme,<institution>Oxford University Clinical Research Unit</institution>
, Ho Chi Minh City,<country>Vietnam</country>
;</nlm:aff>
<country xml:lang="fr">Viêt Nam</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation><nlm:aff id="aff18">Division of Infectious Diseases, Department of Medicine,<institution>University of California, San Francisco</institution>
,<addr-line>CA 94118-6215</addr-line>
;</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Nhu, Nguyen Thi Khanh" sort="Nhu, Nguyen Thi Khanh" uniqKey="Nhu N" first="Nguyen Thi Khanh" last="Nhu">Nguyen Thi Khanh Nhu</name>
<affiliation wicri:level="1"><nlm:aff id="aff7">The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme,<institution>Oxford University Clinical Research Unit</institution>
, Ho Chi Minh City,<country>Vietnam</country>
;</nlm:aff>
<country xml:lang="fr">Viêt Nam</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff19">School of Chemistry and Molecular Biosciences,<institution>The University of Queensland</institution>
, Brisbane, QLD 4072,<country>Australia</country>
;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Schultsz, Constance" sort="Schultsz, Constance" uniqKey="Schultsz C" first="Constance" last="Schultsz">Constance Schultsz</name>
<affiliation wicri:level="1"><nlm:aff id="aff7">The Hospital for Tropical Diseases, Wellcome Trust Major Overseas Programme,<institution>Oxford University Clinical Research Unit</institution>
, Ho Chi Minh City,<country>Vietnam</country>
;</nlm:aff>
<country xml:lang="fr">Viêt Nam</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff24">Academic Medical Center,<institution>University of Amsterdam</institution>
, 1012 WX Amsterdam,<country>The Netherlands</country>
;</nlm:aff>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Kuntaman, Kuntaman" sort="Kuntaman, Kuntaman" uniqKey="Kuntaman K" first="Kuntaman" last="Kuntaman">Kuntaman Kuntaman</name>
<affiliation wicri:level="1"><nlm:aff id="aff21">Department of Clinical Microbiology, Dr. Soetomo Academic Hospital – School of Medicine,<institution>Airlangga University</institution>
, Surabaya, Jawa Timur,<country>Indonesia</country>
;</nlm:aff>
<country xml:lang="fr">Indonésie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Newton, Paul N" sort="Newton, Paul N" uniqKey="Newton P" first="Paul N." last="Newton">Paul N. Newton</name>
<affiliation wicri:level="1"><nlm:aff id="aff4">Nuffield Department of Clinical Medicine, Centre for Tropical Medicine,<institution>University of Oxford</institution>
, OX3 7BN Oxford,<country>United Kingdom</country>
;</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation><nlm:aff id="aff9">Lao-Oxford-Mahosot Hospital Wellcome Trust Research Unit, Microbiology Laboratory,<institution>Mahosot Hospital</institution>
, Vientiane,<country>Lao People’s Democratic Republic</country>
;</nlm:aff>
<wicri:noCountry code="nlm country">Lao People’s Democratic Republic</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Moore, Catrin E" sort="Moore, Catrin E" uniqKey="Moore C" first="Catrin E." last="Moore">Catrin E. Moore</name>
<affiliation wicri:level="1"><nlm:aff id="aff4">Nuffield Department of Clinical Medicine, Centre for Tropical Medicine,<institution>University of Oxford</institution>
, OX3 7BN Oxford,<country>United Kingdom</country>
;</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation><nlm:aff id="aff9">Lao-Oxford-Mahosot Hospital Wellcome Trust Research Unit, Microbiology Laboratory,<institution>Mahosot Hospital</institution>
, Vientiane,<country>Lao People’s Democratic Republic</country>
;</nlm:aff>
<wicri:noCountry code="nlm country">Lao People’s Democratic Republic</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Strugnell, Richard A" sort="Strugnell, Richard A" uniqKey="Strugnell R" first="Richard A." last="Strugnell">Richard A. Strugnell</name>
<affiliation wicri:level="1"><nlm:aff id="aff2">Department of Microbiology and Immunology,<institution>The University of Melbourne</institution>
, Parkville, VIC 3010, Australia;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>, Parkville, VIC 3010</wicri:regionArea>
<wicri:noRegion>VIC 3010</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff16"><institution>Peter Doherty Institute, The University of Melbourne</institution>
, Parkville, VIC 3010, Australia;</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>, Parkville, VIC 3010</wicri:regionArea>
<wicri:noRegion>VIC 3010</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Thomson, Nicholas R" sort="Thomson, Nicholas R" uniqKey="Thomson N" first="Nicholas R." last="Thomson">Nicholas R. Thomson</name>
<affiliation wicri:level="1"><nlm:aff id="aff11">Pathogen Genomics,<institution>Wellcome Trust Sanger Centre</institution>
, CB10 1SA Cambridge,<country>United Kingdom</country>
;</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff25">Department of Pathogen Molecular Biology,<institution>The London School of Hygiene and Tropical Medicine</institution>
, WC1E 7HT London,<country>United Kingdom</country>
</nlm:aff>
<country xml:lang="fr">Royaume-Uni</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
</analytic>
<series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title>
<idno type="ISSN">0027-8424</idno>
<idno type="eISSN">1091-6490</idno>
<imprint><date when="2015">2015</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Anti-Infective Agents (pharmacology)</term>
<term>Bacterial Proteins (classification)</term>
<term>Bacterial Proteins (genetics)</term>
<term>Cross Infection (microbiology)</term>
<term>Drug Resistance, Multiple, Bacterial (genetics)</term>
<term>Genes, Bacterial (genetics)</term>
<term>Genetic Variation</term>
<term>Genome, Bacterial (genetics)</term>
<term>Genomics (methods)</term>
<term>Humans</term>
<term>Klebsiella Infections (microbiology)</term>
<term>Klebsiella pneumoniae (classification)</term>
<term>Klebsiella pneumoniae (genetics)</term>
<term>Klebsiella pneumoniae (pathogenicity)</term>
<term>Phylogeny</term>
<term>Population Dynamics</term>
<term>Public Health (statistics & numerical data)</term>
<term>Public Health (trends)</term>
<term>Sequence Analysis, DNA</term>
<term>Species Specificity</term>
<term>Virulence (genetics)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN</term>
<term>Animaux</term>
<term>Anti-infectieux (pharmacologie)</term>
<term>Dynamique des populations</term>
<term>Gènes bactériens (génétique)</term>
<term>Génome bactérien (génétique)</term>
<term>Génomique ()</term>
<term>Humains</term>
<term>Infection croisée (microbiologie)</term>
<term>Infections à Klebsiella (microbiologie)</term>
<term>Klebsiella pneumoniae ()</term>
<term>Klebsiella pneumoniae (génétique)</term>
<term>Klebsiella pneumoniae (pathogénicité)</term>
<term>Multirésistance bactérienne aux médicaments (génétique)</term>
<term>Phylogénie</term>
<term>Protéines bactériennes ()</term>
<term>Protéines bactériennes (génétique)</term>
<term>Santé publique ()</term>
<term>Santé publique (tendances)</term>
<term>Spécificité d'espèce</term>
<term>Variation génétique</term>
<term>Virulence (génétique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="classification" xml:lang="en"><term>Bacterial Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Anti-Infective Agents</term>
</keywords>
<keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Klebsiella pneumoniae</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Drug Resistance, Multiple, Bacterial</term>
<term>Genes, Bacterial</term>
<term>Genome, Bacterial</term>
<term>Klebsiella pneumoniae</term>
<term>Virulence</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Gènes bactériens</term>
<term>Génome bactérien</term>
<term>Klebsiella pneumoniae</term>
<term>Multirésistance bactérienne aux médicaments</term>
<term>Protéines bactériennes</term>
<term>Virulence</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Genomics</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Infection croisée</term>
<term>Infections à Klebsiella</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Cross Infection</term>
<term>Klebsiella Infections</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Klebsiella pneumoniae</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Klebsiella pneumoniae</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Anti-infectieux</term>
</keywords>
<keywords scheme="MESH" qualifier="statistics & numerical data" xml:lang="en"><term>Public Health</term>
</keywords>
<keywords scheme="MESH" qualifier="tendances" xml:lang="fr"><term>Santé publique</term>
</keywords>
<keywords scheme="MESH" qualifier="trends" xml:lang="en"><term>Public Health</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Genetic Variation</term>
<term>Humans</term>
<term>Phylogeny</term>
<term>Population Dynamics</term>
<term>Sequence Analysis, DNA</term>
<term>Species Specificity</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term>
<term>Animaux</term>
<term>Dynamique des populations</term>
<term>Génomique</term>
<term>Humains</term>
<term>Klebsiella pneumoniae</term>
<term>Phylogénie</term>
<term>Protéines bactériennes</term>
<term>Santé publique</term>
<term>Spécificité d'espèce</term>
<term>Variation génétique</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><title>Significance</title>
<p><italic>Klebsiella pneumoniae</italic>
 is rapidly becoming untreatable using last-line antibiotics. It is especially problematic in hospitals, where it causes a range of acute infections. To approach controlling such a bacterium, we first must define what it is and how it varies genetically. Here we have determined the DNA sequence of <italic>K</italic>
. <italic>pneumoniae</italic>
 isolates from around the world and present a detailed analysis of these data. We show that there is a wide spectrum of diversity, including variation within shared sequences and gain and loss of whole genes. Using this detailed blueprint, we show that there is an unrecognized association between the possession of specific gene profiles associated with virulence and antibiotic resistance and the differing disease outcomes seen for <italic>K</italic>
. <italic>pneumoniae</italic>
.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>Australie</li>
<li>France</li>
<li>Indonésie</li>
<li>Pays-Bas</li>
<li>Royaume-Uni</li>
<li>Singapour</li>
<li>Viêt Nam</li>
</country>
</list>
<tree><noCountry><name sortKey="Mensink, Martijn" sort="Mensink, Martijn" uniqKey="Mensink M" first="Martijn" last="Mensink">Martijn Mensink</name>
<name sortKey="Whitehouse, Chris A" sort="Whitehouse, Chris A" uniqKey="Whitehouse C" first="Chris A." last="Whitehouse">Chris A. Whitehouse</name>
</noCountry>
<country name="Australie"><noRegion><name sortKey="Holt, Kathryn E" sort="Holt, Kathryn E" uniqKey="Holt K" first="Kathryn E." last="Holt">Kathryn E. Holt</name>
</noRegion>
<name sortKey="Cao, Hanwei" sort="Cao, Hanwei" uniqKey="Cao H" first="Hanwei" last="Cao">Hanwei Cao</name>
<name sortKey="Cao, Hanwei" sort="Cao, Hanwei" uniqKey="Cao H" first="Hanwei" last="Cao">Hanwei Cao</name>
<name sortKey="Edwards, David J" sort="Edwards, David J" uniqKey="Edwards D" first="David J." last="Edwards">David J. Edwards</name>
<name sortKey="Gorrie, Claire" sort="Gorrie, Claire" uniqKey="Gorrie C" first="Claire" last="Gorrie">Claire Gorrie</name>
<name sortKey="Gorrie, Claire" sort="Gorrie, Claire" uniqKey="Gorrie C" first="Claire" last="Gorrie">Claire Gorrie</name>
<name sortKey="Gorrie, Claire" sort="Gorrie, Claire" uniqKey="Gorrie C" first="Claire" last="Gorrie">Claire Gorrie</name>
<name sortKey="Holt, Kathryn E" sort="Holt, Kathryn E" uniqKey="Holt K" first="Kathryn E." last="Holt">Kathryn E. Holt</name>
<name sortKey="Jenney, Adam" sort="Jenney, Adam" uniqKey="Jenney A" first="Adam" last="Jenney">Adam Jenney</name>
<name sortKey="Jenney, Adam" sort="Jenney, Adam" uniqKey="Jenney A" first="Adam" last="Jenney">Adam Jenney</name>
<name sortKey="Nhu, Nguyen Thi Khanh" sort="Nhu, Nguyen Thi Khanh" uniqKey="Nhu N" first="Nguyen Thi Khanh" last="Nhu">Nguyen Thi Khanh Nhu</name>
<name sortKey="Schultz, Mark B" sort="Schultz, Mark B" uniqKey="Schultz M" first="Mark B." last="Schultz">Mark B. Schultz</name>
<name sortKey="Strugnell, Richard A" sort="Strugnell, Richard A" uniqKey="Strugnell R" first="Richard A." last="Strugnell">Richard A. Strugnell</name>
<name sortKey="Strugnell, Richard A" sort="Strugnell, Richard A" uniqKey="Strugnell R" first="Richard A." last="Strugnell">Richard A. Strugnell</name>
<name sortKey="Wilksch, Jonathan" sort="Wilksch, Jonathan" uniqKey="Wilksch J" first="Jonathan" last="Wilksch">Jonathan Wilksch</name>
<name sortKey="Wilksch, Jonathan" sort="Wilksch, Jonathan" uniqKey="Wilksch J" first="Jonathan" last="Wilksch">Jonathan Wilksch</name>
</country>
<country name="Royaume-Uni"><noRegion><name sortKey="Wertheim, Heiman" sort="Wertheim, Heiman" uniqKey="Wertheim H" first="Heiman" last="Wertheim">Heiman Wertheim</name>
</noRegion>
<name sortKey="Connor, Thomas R" sort="Connor, Thomas R" uniqKey="Connor T" first="Thomas R." last="Connor">Thomas R. Connor</name>
<name sortKey="Connor, Thomas R" sort="Connor, Thomas R" uniqKey="Connor T" first="Thomas R." last="Connor">Thomas R. Connor</name>
<name sortKey="Dance, David" sort="Dance, David" uniqKey="Dance D" first="David" last="Dance">David Dance</name>
<name sortKey="Moore, Catrin E" sort="Moore, Catrin E" uniqKey="Moore C" first="Catrin E." last="Moore">Catrin E. Moore</name>
<name sortKey="Newton, Paul N" sort="Newton, Paul N" uniqKey="Newton P" first="Paul N." last="Newton">Paul N. Newton</name>
<name sortKey="Thomson, Nicholas R" sort="Thomson, Nicholas R" uniqKey="Thomson N" first="Nicholas R." last="Thomson">Nicholas R. Thomson</name>
<name sortKey="Thomson, Nicholas R" sort="Thomson, Nicholas R" uniqKey="Thomson N" first="Nicholas R." last="Thomson">Nicholas R. Thomson</name>
<name sortKey="Zadoks, Ruth N" sort="Zadoks, Ruth N" uniqKey="Zadoks R" first="Ruth N." last="Zadoks">Ruth N. Zadoks</name>
</country>
<country name="Viêt Nam"><noRegion><name sortKey="Baker, Stephen" sort="Baker, Stephen" uniqKey="Baker S" first="Stephen" last="Baker">Stephen Baker</name>
</noRegion>
<name sortKey="Dao, Trinh Tuyet" sort="Dao, Trinh Tuyet" uniqKey="Dao T" first="Trinh Tuyet" last="Dao">Trinh Tuyet Dao</name>
<name sortKey="Minh, Vien Le" sort="Minh, Vien Le" uniqKey="Minh V" first="Vien Le" last="Minh">Vien Le Minh</name>
<name sortKey="Nguyen, Kinh Van" sort="Nguyen, Kinh Van" uniqKey="Nguyen K" first="Kinh Van" last="Nguyen">Kinh Van Nguyen</name>
<name sortKey="Nguyen, Trung Vu" sort="Nguyen, Trung Vu" uniqKey="Nguyen T" first="Trung Vu" last="Nguyen">Trung Vu Nguyen</name>
<name sortKey="Nhu, Nguyen Thi Khanh" sort="Nhu, Nguyen Thi Khanh" uniqKey="Nhu N" first="Nguyen Thi Khanh" last="Nhu">Nguyen Thi Khanh Nhu</name>
<name sortKey="Schultsz, Constance" sort="Schultsz, Constance" uniqKey="Schultsz C" first="Constance" last="Schultsz">Constance Schultsz</name>
</country>
<country name="Singapour"><noRegion><name sortKey="Hsu, Li Yang" sort="Hsu, Li Yang" uniqKey="Hsu L" first="Li Yang" last="Hsu">Li Yang Hsu</name>
</noRegion>
</country>
<country name="Pays-Bas"><noRegion><name sortKey="Severin, Juliette" sort="Severin, Juliette" uniqKey="Severin J" first="Juliëtte" last="Severin">Juliëtte Severin</name>
</noRegion>
<name sortKey="Schultsz, Constance" sort="Schultsz, Constance" uniqKey="Schultsz C" first="Constance" last="Schultsz">Constance Schultsz</name>
</country>
<country name="France"><noRegion><name sortKey="Brisse, Sylvain" sort="Brisse, Sylvain" uniqKey="Brisse S" first="Sylvain" last="Brisse">Sylvain Brisse</name>
</noRegion>
</country>
<country name="Indonésie"><noRegion><name sortKey="Kuntaman, Kuntaman" sort="Kuntaman, Kuntaman" uniqKey="Kuntaman K" first="Kuntaman" last="Kuntaman">Kuntaman Kuntaman</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002D45 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002D45 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Asie
   |area=    AustralieFrV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     PMC:4500264
   |texte=   Genomic analysis of diversity, population structure, virulence, and antimicrobial resistance in Klebsiella pneumoniae, an urgent threat to public health
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:26100894" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a AustralieFrV1

This area was generated with Dilib version V0.6.33.
Data generation: Tue Dec 5 10:43:12 2017. Site generation: Tue Mar 5 14:07:20 2024

Serveur d'exploration sur les relations entre la France et l'Australie

Genomic analysis of diversity, population structure, virulence, and antimicrobial resistance in Klebsiella pneumoniae, an urgent threat to public health

Genomic analysis of diversity, population structure, virulence, and antimicrobial resistance in Klebsiella pneumoniae, an urgent threat to public health

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration sur les relations entre la France et l'Australie
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.